5 Essential Elements For medicalesthe-bisearch
5 Essential Elements For medicalesthe-bisearch
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Treatment of genomic DNA with bisulfite and subsequent PCR of the location of curiosity delivers PCR products wherein originally unmethylated cytosines take place as thymines and methylated cytosines as cytosines. Subcloning and sequencing of the PCR products
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In order to ameliorate the effectiveness of PCR, we created a brand new primer-design application termed BiSearch, accessible about the Web. It's got the unique property of analyzing the primer pairs for mispriming web pages on the bisulfite-treated genome and determines possible non-particular amplification items using a new lookup algorithm. The options of primer-style and Evaluation for mispriming websites can be used sequentially or sep...
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